Chokoleti ndi chakudya chodziwika bwino, koma nyemba za koko zomwe zimapangidwira chokoleti kapena masiwiti ena nthawi zina zimakhala ndi kukoma kapena fungo losasangalatsa, zomwe zimapangitsa kuti chomalizacho chiwonongeke.Komabe, pafupifupi palibe amene akudziwa kuti mankhwala okhudzana ndi fungo ili ndi chiyani.Nyemba za koko zikathiridwa bwino, zimakhala ndi fungo lokoma lamaluwa.Koma ngati nayonso mphamvu ikapita molakwika, kapena kusungirako sikuli bwino, ndipo tizilombo tating'onoting'ono timakula pamenepo, timatulutsa fungo losasangalatsa.Ngati nyemba za khofizi zimalowa mukupanga, chokoleti chotsatira chidzatulutsa fungo losasangalatsa, lomwe pamapeto pake lidzabweretsa madandaulo a ogula ndikukumbukira.Ofufuza adagwiritsa ntchito chromatography ya gasi, kuyesa kununkhiza, ndi ma spectrometry ambiri kuti azindikire mamolekyu 57 omwe amapanga fungo la nyemba wamba za cocoa ndi nyemba za koko.Pakati pa mankhwalawa, 4 ali ndi zochulukira kwambiri m'masampuli osakometsera.Pambuyo poyesa, gulu lofufuza lidatsimikiza kuti geosmin-yokhudzana ndi fungo la nkhungu ndi beetroot, ndi 3-methyl-1H-indole-yokhudzana ndi fungo la ndowe ndi mipira ya camphor, imayambitsa fungo la nkhungu ndi musty la chinthu chachikulu cha cocoa.Pomaliza, adapeza kuti geosmin imakhala makamaka mu mankhusu a nyemba ndipo imatha kuchotsedwa pokonza;3-methyl-1H-indole makamaka ili kunsonga kwa nyemba, yomwe imapangidwa kukhala chokoleti.